Larvicidal study in Aedes aegypti (Linnaeus, 1762), cytotoxic and phytochemical of the crude ethanolic extract of Mikania lindleyana DC.

Resumo

Mikania lindleyana is a plant belonging to the Asteraceae family, popularly known as Sucuriju, it is a large vine, perennial used in folk medicine as anti-inflammatory, analgesic, healing, diuretic, antihypertensive in the treatment against varicose veins and acne. Plants of this family present themselves as potential biocides in the control of Aedes aegypti. The objective of this research was to evaluate the larvicidal activity against Aedes aegypti, the phytochemical and cytotoxicity study of the crude ethanolic extract of Mikania lindleyana. The phytochemical screening of the crude ethanolic extract showed saponins, azulenes and depsides, these secondary metabolites that may justify some biological activities of the plant species. Larvicidal activity showed an LC50 of 146.32 µg.mL-1, the extract was able to cause the death of individuals in the concentration 100 µg.mL-1 in 48 hours of contact with the larvae. The extract against Artemia salina was not able to cause the death of any metanáuplium in the tested concentrations. This research indicated the presence of secondary metabolites saponins, azulenes and depsides in the crude ethanolic extract, indicated larvicidal activity against Aedes aegypti larvae probably caused by the presence of saponins and did not show cytotoxicity against Artemia salina indicating low toxicity of the extract.